SANDHYA SHRESTHA

Currently , working as registered Medical Laboratory Technologist in laboratory of tertiary level hospital in microbiology department.

• Optimized workload distribution among team members, ensuring timely completion of tasks without compromising quality.
• Enhanced laboratory efficiency by streamlining specimen processing and implementing effective quality control measures.
• Collaborated with interdisciplinary healthcare teams for improved patient outcomes, sharing critical lab findings and insights.
• Performed comprehensive analysis of patient samples and generated detailed reports to inform medical staff of results.
• Completed comprehensive diagnostic testing of blood, urine and other body fluids.
• Prepared reports to document laboratory results and findings.
• Promoted a culture of continuous improvement within the laboratory team by leading regular performance reviews and goal-setting sessions.

• Performing diagnosis of various infectious diseases (Mycobacterium tuberculosis, HBV, HCV viral load and HPV, etc.), mutational disorders (JAK-2, Gilbert Syndrome, NAFLD, Thalassemia, Sickle cell, HLA B27, etc.), and cancer genetics (EGFR, ALK)
• Worked in COVID molecular laboratory along with influenza detection
• Collecting, processing, and testing of specimens as per appropriate PCR techniques and clinical protocols, gathering patient’s vitals and patient history
• Extraction and handling of extracted samples using Real-Time and Conventional PCR techniques, gel electrophoresis of PCR product and analysis
• Operating lab equipment’s including real-time PCR (Qiagen: Step-one PCR) and conventional PCR (Applied Biosystem), maintaining quality control, analysis of test result and report dispatch
• Worked as frontline during COVID Pandemic-2019, diagnosing Covid-19, processing thousands of samples
• Handling of daily maintenance, calibrations, and quality control of each biochemistry profile test
• Operation of various test parameters of biochemistry like Liver Function Test, Renal Function Test, Lipid Profile Test,
• In semi-automated and fully-automated biochemistry machine
• Analyzing and correlating reports with patient history
• Handling daily maintenance, quality control Microscopy (Wright stain)
• Perform Manual cell count i.e., Total blood count, Differential count, Platelet count, etc
• Operation of fully automated coulter (Beckman Coulter) with Laboratory Information System
• Perform daily maintenance, calibration, and quality control of each parameter like HBsAg, HIV, HCV Detection of levels of hormones like prolactin, procalcitonin, Thyroid Function Test
• Detection of vitamins like folic acid, vitamin B12, vitamin D, etc
• Using CLIA E411
• Operation of automated ELISA machine (CHORUS) for specialized infectious and autoimmune panel
• Perform infectious panel test such as TORCH panel, Clostridium difficle toxin, Helicobacter pylori, Galactomannan, etc
• Perform autoimmune panel test such as ANA, Anti-ccp, ds-DNA, SCL-70, Total IgE, etc
• Processing and reporting of ENA panel, Autoimmune Liver panel, Allergic panel, etc
• By ImmunoBlot Technique
• Perform microscopy (Gram stain, ZN-stain, Giemsa stain, etc.)
• Do culture for bacteria, fungus
• Using different techniques Using of automated BACTEC system for blood culture Biochemical test and conduct associated analysis
• Anti-biotic sensitivity test
• Assist pathologist in grossing of tissue
• Perform tissue processing, section cutting, mounting of tissue
• Staining of slides for PAP smear, Liquid Based Cytology (LBC), Fine Needle Aspiration Cytology specimens

• Clinical posting at National Public Health Laboratory (NPHL), Kathmandu
• Clinical posting at Bharatpur Cancer Hospital, Chitwan for three weeks
• Clinical posting at Nobel hospital, Kathmandu for four months
• Clinical posting at Kantipur hospital, Kathmandu for four months
• Clinical posting at Nepal Red Cross Society Blood Bank, Kathmandu for a week